Effects of excessive nitrogen fertilizer and soil moisture deficiency on antioxidant enzyme system and osmotic adjustment in tomato seedlings

Yujie Zhang, Shuangen Yu, Zijia Li, Tingting Chang, Qicong Xu, Huilian Xu, Jie Zhang

Abstract


Long-term excessive application of nitrogen fertilizer induces secondary salinization of soil, which results in inhibiting plant growth. In addition, soil moisture deficiency also affects plant growth. To investigate the effects of excessive nitrogen fertilizer and soil moisture deficiency on the antioxidant enzyme system, plant water relations analyzed through pressure-volume (P-V) curve, and photosynthetic light response parameters in tomato (Solanum lycopersicum L. Myoko) seedlings, an indoor experiment of about 50 d was conducted using two irrigation water amounts based on field capacity (soil moisture deficiency: 50%-80%; adequate water: 70%-80%), two nitrogen fertilizer rates (moderate nitrogen; excessive nitrogen fertilizer: 0.585 g/pot) and two types of irrigation water (tap water and microbial diluent). The results showed that excessive nitrogen fertilizer (N) and soil moisture deficiency (W) reduced the biomass of tomato seedlings. In comparison to CK (combination of adequate water and tap water quality), microbial dilution (EM) increased plant biomass by 5.2%. Also, the nitrogen application increased chlorophyll relative contents (SPAD). The maximum net photosynthetic rate (Pc) decreased with nitrogen application and increased with EM application and irrigation amount. Excessive nitrogen application increased the plant nitrate reductase activity (NR). The plant NR in the N treatment showed a 13.0% increase compared to CK, and the plant NR in the treatment of nitrogen application with water deficiency (WN) increased 34.0% compared to water deficiency (W). After applying excessive nitrogen, N, EM-N, WN, EM-WN respectively increased the plant nitrate reductase activity by 13.0%, 22.9%, 34.0%, and 28.6%, compared with the corresponding treatment with moderate nitrogen (i.e., CK, EM, W and EM-W). In addition, the activities of antioxidant enzymes [superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT)] in four treatments of nitrogen application (N, EM-N, WN, EM-WN) also increased significantly. Both soil moisture and nitrogen fertilizer significantly affect the parameters of osmotic adjustment, which is manifested in the reduction of osmotic potential (πFT), and the increase in the osmotic concentration (Cosm) and concentration difference (ΔCosm). But the decrease in the relative water content of apoplast (ζap) indicated that water deficiency and excessive nitrogen reduced the water absorption and water retention capacity of tomatoes to a certain extent. In conclusion, excessive nitrogen application and soil moisture deficiency inhibit plant growth significantly in this experiment. Meanwhile, microbial dilution can alleviate excessive nitrogen fertilizer and water stress to some extent, but the effect was not significant.
Keywords: antioxidant enzyme, nitrogen, osmotic adjustment, pressure-volume curve, stress, tomato, water
DOI: 10.25165/j.ijabe.20221502.5555

Citation: Zhang Y J, Yu S E, Li Z J, Chang T T, Xu Q C, Xu H L, et al. Effects of excessive nitrogen fertilizer and soil moisture deficiency on antioxidant enzyme system and osmotic adjustment in tomato seedlings. Int J Agric & Biol Eng, 2022; 15(2): 127–134.

Keywords


antioxidant enzyme, nitrogen, osmotic adjustment, pressure-volume curve, stress, tomato, water

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References


Boyer J S, Byrne P, Cassman K, Cooper M, Delmer D, Greene T, et al. The U.S. drought of 2012 in perspective: A call to action. Global Food Security, 2013; 3(2): 139–143.

Trenberth K E, Dai A, Schrier G, Jones P D, Barichivich J, Briffa K R, et al. Global warming and changes in drought. Nature Climate Change, 2014; 4: 17–22.

Wetherald R T, Manabe S. Simulation of hydrologic changes associated with global warming. Journal of Geophysical Research, 2002; 107(D19): 7–15.

Chang T T, Zhang Y J, Zhang Z Y, Shao X H, Wang W N, Zhang J, et al. Effects of irrigation regimes on soil NO3--N, electrical conductivity and crop yield in plastic greenhouse. International Journal of Agricultural and Biological Engineering, 2019; 12(1): 109–115.

Schindler D W, Donahue W F. An impending water crisis in Canada’s western prairie provinces. Proceedings of the National Academy of Sciences of the United States of America, 2006; 7: 7210–7216.

Ju X, Liu X, Zhang F, Roelcke M. Nitrogen fertilization, soil nitrate accumulation, and policy recommendations in several agricultural regions of China. AMBIO, 2004; 33(6): 300–305.

Xin K. Four characteristics of current facility gardening. Northwest Horticulture, 2018; 4: 33–34. (in Chinese)

Yang L, Huang B, Mao M, Yao L, Niedermann S, Hu W, Chen Y. Sustainability assessment of greenhouse vegetable farming practices from environmental, economic, and socio-institutional perspectives in China. Environmental Science and Pollution Research, 2016; 23(17): 17287–17297.

Ju X, Kou C, Zhang F, Christie P. Nitrogen balance and groundwater nitrate contamination: comparison among three intensive cropping systems on the North China Plain. Environment Pollution, 2006; 43: 117–125.

Ning D F, Qing A Z, Liu Z D, Zhang J Y, Liu Z J, Zhao B, et al. Effects of water nitrogen supply by drip irrigation on yield, nitrate nitrogen and water nitrogen utilization efficiency of summer maize. Journal of Irrigation and Drainage, 2019; 38(9): 28–35. (in Chinese)

Yang H W, Ji J W, Wang C, Zhang L Y, Wang X D, Song P, et al. Micro-nondestructive detection of the moisture and ion of rice seeds during germination under salt stress. International Journal of Agricultural and Biological Engineering, 2019; 12(2): 103–110.

Du Z L, Liu S F, Xiao X P, Yang G L, Ren T S. Soil physical quality as influenced by long-term fertilizer management under an intensive cropping system. Int J Agric & Biol Eng, 2009; 2(1): 19–27.

Li Y Q, Zhao B Q, Li Z. Research progress on combined organic and inorganic fertilization system. Journal of Agriculture, 2017; 7(7): 22–30. (in Chinese)

Goldhamer D, Beede R. Regulated deficit irrigation effects on yield, nut quality and water-use efficiency of mature pistachio trees. Journal of Pomology and Horticultural Science, 2004; 79(4): 538–545.

Kang S Z, Shi W J, Zhang J H. An improved water-use efficiency for maize grown under regulated deficit irrigation. Field Crops Research, 2000; 67(3): 207–214.

Zhao Q Y, Zhang G C. Effect of duration of greenhouse soil on growth and development of tomato. Journal of Jilin Agricultural University, 2013; 35(5): 541–546, 551. (in Chinese)

Shao X H, Tan M, Jiang P, Cao W. Effect of EM bokashi application on control of secondary soil salinization. Water Science and Engineering, 2008; 1(4): 99–106.

Meng F Q, Wu W L, Xin D H. Relationship between soil organic matter, nutrient changes and crop yield in high yield farmland. Journal of Plant Nutrition and Fertilizer, 2000; 6(4): 370–374. (in Chinese)

Yao N, Zhou Y G, Song L B, Liu J, Li Y, Wu S F, el al. DSSAT-CERES-Wheat model was tested under different water stress conditions. Transactions of the Chinese Society of Agricultural Engineering, 2015; 31(12): 138–150. (in Chinese)

Panigrahi B. A Handbook on Irrigation and Drainage. New India Publishing Agency, New Delhi, 201; 601p.

Panigrahi B, Panda, S N, Roy D P. Water use and yield response of tomato as influenced by drip and furrow irrigation. International Agricultural Engineering Journal, 2010; 19(1): 19–30.

Zhang J, Sui X, Li B, Su B L, Li J M, Zhou D X. An improved water-use efficiency for winter wheat grown under reduced irrigation. Field Crops Research, 1998; 59(2): 91–98.

Fang D P, Zhang F C, Li J, Wang H D, Xiang Y Z, Zhang Y. Effects of irrigation and fertilizer on yield and quality of cucumber in greenhouse. Chinese Journal of Applied Ecology, 2015; 26(6): 1735–1742. (in Chinese)

Xing Y Y, Zhang F C, Wu L F, Fan J L, Zhang Y, Li J. Based on the utilization efficiency of water and fertilizer of tomato yield and quality to determine the suitable amount of fertilizer for drip irrigation. Transactions of the CSAE, 2015; 31(S1): 110–121. (in Chinese).

Sauvé S, Desrosiers M. A review of what is an emerging contaminant. Chemistry Central Journal, 2014; 8: 15. doi: 10.1186/1752-153X-8-15

Al-Omran A M, Al-Harbi A R, Wahb-Allah M A. Impact of irrigation water quality, irrigation systems, irrigation rates and soil amendments on tomato production in sandy calcareous soil. Turkish Journal of Agriculture and Forestry, 2010; 34: 59–73.

Mcheik A, Awad A, Fadel A, Mounzer C, Nasreddine S. Effect of irrigation water quality on the microbial contamination of fresh vegetables in the Bekaa Valley, Lebanon. American Journal of Agriculture and Forestry, 2018; 6(6): 191–197.

Olle M, Williams I H. Effective microorganisms and their influence on vegetable production – a review. The Journal of Horticultural Science and Biotechnology, 2013; 88 (4): 380–386.

Xu H L, Xu Q C, Li F L, Feng Y Z, Qin F F, Fang W. Applications of xerophytophysiology in plant production—LED blue light as a stimulus improved the tomato crop. Scientia Horticulturae, 2012; 148: 190–196.

Xu H L, Qin F F, Xu Q C, Tan J Y, Liu G M. Applications of xerophytophysiology in plant production –The potato crop improved by partial root zone drying of early season but not whole season. HortScience, 2011; 129: 528–534.

Jones H G. Plant and microclimate: A quantitative approach to environmental plant physiology. Cambridge University Press, London, UK, 1992; 456p.

Chang T T, Zhang Y J, Xu H L, Shao X H, Xu Q C, Li F L, et al. Osmotic adjustment and up-regulation expression of stress-responsive genes in tomato induced by soil salinity resulted from nitrate fertilization. Int J Agric & Biol Eng, 2018; 11(3): 126–136.

Elfstrand S, Bath B, Mortenson A. Influence of various forms of green manure amendment on soil microbial community composition, enzyme activity and nutrient levels in leek. Applied Soil Ecology, 2007; 36(1): 70–82.

Di X Y, Wang M B, Chen J W. Study on water parameters of poplar clone PV curve. Acta Botanica Boreali-Occidentalia Sinica, 2007; 27(1): 98–103. (in Chinese)

Pryor S C, Barthelmie R J, Schoof J T. High-resolution projections of climate-related risks for the Midwestern USA. Climate Research, 2013; 56(1): 61–79.

Schindler D W. Lakes as sentinels and integrators for the effects of climate change on watersheds, airsheds, and landscapes. Limnol Oceanography, 2009; 54(6): 2349–2358.

Bita C E, Gerats T. Plant tolerance to high temperature in a changing environment: Scientific fundamentals and production of heat stress-tolerant crops. Frontiers in Plant Science, 2013; 4: 273. doi: 10.3389/fpls.2013.00273.

Gourdji S M, Sibley A M, Lobell D B. Global crop exposure to critical high temperatures in the reproductive period: Historical trends and future projections. Environmental Research Letters, 2013; 8(2): 24–41.

Campoe O C, Stape J L, Albaugh T J, Allen H L, Fox T R, Rubilar R, Binkley D. Fertilization and irrigation effects on tree level aboveground net primary production, light interception and light use efficiency in a loblolly pine plantation. Forest Ecology and Management, 2013; 288: 43–48.

Peri P L, Moot D J, Mcneil D L, Varella A C, Lucas R J. Modelling net photosynthetic rate of field-grown cocksfoot leaves under different nitrogen, water and temperature regimes. Grass and Forage Science, 2002; 57(1): 61–71.

Lin X, Wang D, Gu S B, White P J, Han K, Zhou J, Jin S P. Effect of supplemental irrigation on the relationships between leaf ABA concentrations, tiller development and photosynthate accumulation and remobilization in winter wheat. Plant Growth Regulation, 2016; 79(3): 331–343.

Chastain D R, Snider J L, Collins G D, Perry CD, Whitaker J, Byrda S A. Water deficit in field-grown Gossypium hirsutum primarily limits net photosynthesis by decreasing stomatal conductance, increasing photorespiration, and increasing the ratio of dark respiration to gross photosynthesis. Journal of Plant Physiology, 2014; 171(17): 1576–1585.

Cabello-Pasini A, Macías-Carranza V, Abdala R, Korbee N, Figueroa F. Effect of nitrate concentration and UVR on photosynthesis, respiration, nitrate reductase activity, and phenolic compounds in Ulva rigida (Chlorophyta). Journal of Applied Phycology, 2011; 23(3): 363–369.

Ahmad S, Fazli I S, Jamal A, Iqbal M, Abdin M Z. Interactive effect of sulfur and nitrogen on nitrate reductase and ATP-sulfurylase activities in relation to seed yield from Psoralea corylifolia L. Journal of Plant Biology, 2007; 50(3): 351–357.




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