Effects of strain, nutrients concentration and inoculum size on microalgae culture for bioenergy from post hydrothermal liquefaction wastewater

Zhang Li, Lu Haifeng, Yuanhui Zhang, Ma Shanshan, Li Baoming, Liu Zhidan, Duan Na, Liu Minsheng, Si Buchun, Lu Jianwen

Abstract


Cultivating microalgae in post hydrothermal liquefaction wastewater (PHWW) offers many benefits, including nutrients recovery and reuse, wastewater purification and biomass production. However, the high nutrients concentration and toxic substances in PHWW undermine the efficiency of biomass production and nutrient recovery. This study aimed to investigate the effects of the microalgae strains, initial nutrients concentrations and inoculum sizes on biomass production and nutrient recovery using PHWW as the cultivation medium. Results indicated that both biomass production and nutrients recovery were successfully improved by using the screened microalgae strain at the desirable initial nutrient concentration with the suggested algae inoculum size. Chlorella vulgaris 1067 probably demonstrated the strongest tolerance ability among the five microalgae strains screened, and performed well in the diluted PHWW, of which initial TN concentration was approximately 500 mg/L. The desirable inoculum size was determined to be 0.103-0.135 g/L. The biomass daily productivity was increased by 15.67-fold (reached 0.13 g/(L·d)). With the above optimal conditions, high biomass production and nutrient recovery from the PHWW to produce microalgae biomass for bioenergy production were achieved.
Keywords: post hydrothermal liquefaction wastewater, microalgae strain screening, inoculum size, initial nutrient concentration, nutrient recovery, biomass production
DOI: 10.3965/j.ijabe.20171002.2882

Citation: Zhang L, Lu H F, Y H Zhang, Ma S S, Li B M, Liu Z D, et al. Effects of strain, nutrients concentration and inoculum size on microalgae culture for bioenergy from post hydrothermal liquefaction wastewater. Int J Agric & Biol Eng, 2017; 10(2): 194–204.

Keywords


post hydrothermal liquefaction wastewater, microalgae strain screening, inoculum size, initial nutrient concentration, nutrient recovery, biomass production

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References


Yu G, Zhang Y H, Schideman L, Funk T, Wang Z C. Distributions of carbon and nitrogen in the products from hydrothermal liquefaction of low-lipid microalgae. Energ Environ Sci, 2011; 4(11): 4587–4595.

Tian C Y, Li B M, Liu Z D, Zhang Y H, Lu H F. Hydrothermal liquefaction for algal biorefinery: Acirical Review. Renew Sust Energ Rev, 2014; 38: 933–950.

Chen W T, Zhang Y H, Zhang J X, Zhang P, Schideman L, Minarick M. Hydrothermal liquefaction of mixed-culture algal biomass from wastewater treatment system into bio-crude oil. Bioresour Technol, 2014; 152: 130–139.

Chen W T, Zhang Y H, Zhang J X, Schideman L, Yu G, Zhang P, et al. Co-liquefaction of swine manure and mixed-culture algal biomass from a wastewater treatment system to produce bio-crude oil. Appl Energ, 2014; 128: 209–216.

Zhou Y, Schideman L, Yu G, Zhang Y H. A synergistic combination of algal wastewater treatment and biofuel production maximized by nutrient and carbon recycling. Energ Environ Sci, 2013; 6: 3765–3779.

Alba L G, Torri C, Fabbri D, Kersten S R A, Brilman D W F. Microalgae growth on the aqueous phase from hydrothermal liquefaction of the same microalgae. Chem Eng J, 2013; 228: 214–223.

Selvaratnam T, Pegallapati A K, Reddy H K, Kanapathipillai N, Nirmalakhandan N, Deng S, et al. Algal biofuels from urban wastewaters: Maximizing biomass yield using nutrients recycled from hydrothermal processing of biomass. Bioresour Technol, 2015; 232–238.

Biller P, Ross A B, Skill S C, Lea-Langton A, Balasundaram B, Hall C, et al. Nutrient recycling of aqueous phase for microalgae cultivation from the hydrothermal liquefaction process. Algal Res, 2012; 1: 70–76.

Jena U, Vaidyanathan N, Chinnasamy S, Das K C. Evaluation of microalgae cultivation using recovered aqueous co-product from thermo chemical liquefaction of algal biomass. Bioresour Technol, 2012; 102: 3380–3387.

Pham M, Schideman L, Scott J, Rajagopalan N, Plewa M J. Chemical and biological characterization of wastewater generated from hydrothermal liquefaction of Spirulina. Environ Sci Technol, 2012; 47(4): 2131–2138.

Zhou Y, Schideman L, Zhang Y H, Yu G, Wang Z C, Pham M. Resolving bottlenecks in current algal wastewater treatment paradigms: A synergistic combination of low-lipid algal wastewater treatment and hydrothermal liquefaction for large-scale biofuel production. Energ Water, 2011; 347–361.

Tommaso G, Chen W T, Li P, Schideman L, Zhang Y H. Chemical characterization and anaerobic biodegradability of hydrothermal liquefaction aqueous products from mixed-culture wastewater algae. Bioresour Technol, 2015; 178: 139–146.

Doucha J, Lívanský K. Production of high-density Chlorella culture grown in fermenters. J Appl Phycol, 2011; 24: 35–43.

Hu B, Min M, Zhou W G, Du Z Y, Mohr M, Chen P, et al. Enhanced mixotrophic growth of microalga Chlorella sp. on pretreated swine manure for simultaneous biofuel feedstock production and nutrient removal. Bioresour Technol, 2012; 126: 71–79.

Wang H, Xiong H, Hui Z, ZengX. Mixotrophic Cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. Bioresour Technol, 2012; 104: 215–220.

Yang F F, He S B, Li M F, Dai D L, Chen X C. Mixotrophic capability and its effect on the growth of Micocystic aeruginosa. J Agro-Environ Sci, 2012; 31: 1003–1008.

Girard J M, Roy M L, Hafsa M B, Gagnon J, Faucheux N, Heitza M, et al. Mixotrophic cultivation of green microalgae Scenedesmus obliquus on cheese whey permeate for biodiesel production. Algal Res, 2014; 5: 241–248.

Muñoz R, Köllner C, Guieysse B, Mattiasson B. Salicylate biodegradation by various algal-bacterial consortia under photosynthetic oxygenation. Biotechnol Lett, 2003; 25(22): 1905–1911.

Tan X, Kong F X, Zeng Q F, Cao H S, Qian S Q, Zhang M. Seasonal variation of Microcystis in Lake Taihu and its relationships with environmental factors. J Environ Sci, 2009; 21: 892–899.

Si Y B, Yue Y D, Wu Z P, Wang R Y, Deng D P. Bioaccumulation and biodegration of phenol by the algae Microcystis aeruginosakutz. J Anhui Agri University, 2000; 27(3): 269–271.

Gao Y J, Zhou P J, Shen H, Zhou X, Song L R, Shen Y W, et al. Study on biological effects of amphetamine on growth of Microcystis. Environ Sci Technol, 2004; 27(6): 1, 2, 57. (in Chinese)

Lou C, Huang S Q, Xu C. Study of the removal of environmental endocrine disruptor 17 β-estradiol by Microcystis aeruginosa. J Zhejiang University Technol, 2012; 40(1): 25–29.

Richmond A. Biological principals of mass cultivation. In: Richmond A, editor. Handbook of microalgal culture: biotechnology and applied phycology. Ames: Iowa State Press, a Blackwell Publishing Company, 2004. pp. 125–177.

Markou G, Vandamme D, Muylaert K. Ammonia inhibition on Arthro spiraplatensis in relation to the initial biomass density and pH. Bioresour Technol, 2014; 166: 259–265.

Li H, Liu Z D, Zhang Y H, Li B M, Lu H F, Duan N. Conversion efficiency and oil quality of low-lipid high-protein and high-lipid low-protein microalgae via hydrothermal liquefaction. Bioresour Technol, 2014; 154: 322–329.

APHA. American Public Health Association, standard methods for examination of water and wastewater, 21st ed. Washington DC, 2005.

Zhang L, Lu H F, Zhang Y H, Li B M, Liu Z D, Duan N. Nutrient recovery and biomass production by cultivating Chlorella vulgaris 1067 from four Types of post-hydrothermal liquefaction wastewater. J Appl Phycol, 2015.

Lee Y K, Shen H. Basic culturing techniques. In: Richmond A, editor. Handbook of microalgal culture: biotechnology and applied phycology. Ames: Iowa State Press, a Blackwell Publishing Company, 2004; pp. 40–56.

Lineweaver H and Burk D. The determination of enzyme dissociation constants. J Am Chem Soc, 1934; 56: 658–666.

Lai L W, Teo C L, Wahidin S, Annuar M S M. Determination of enzyme kinetic parameters on sago starch hydrolysis by linezrized graphical methods. Malays J Anal Sci, 2014; 18: 527–533.

Qu C B, Wu Z Y, Shi X M. Phosphate assimilation by Chlorella and adjustment of phosphate concentration in basal medium for its Cultivation. Biotechnol Lett, 2008; 30: 1735–1740.

Lee Y K. Basic culturing techniques. In: Richmond A, editor. Handbook of microalgal culture: biotechnology and applied phycology. Ames: Iowa State Press, a Blackwell Publishing Company. 2004. pp 116–124.

Biller P, Ross A B. Potential yields and properties of oil from the hydrothermal liquefaction of microalgae with different biochemical content. Bioresour Technol, 2011; 102: 215–225.

Shi X M, Zhang X W, Chen F. Heterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources. Enzyme Microb Technol, 2000; 27: 312–318.

Zheng X Y, Yan J, Yu X R, Gu Y J, Zhu Y Q, Yang Y F. Influence of nitrogen and phosphorus concentrations on the growth characteristics of Microcystisaeruginosa. J East China Normal University (Natural Science), 2012; 1: 12–18.

Wang J H, Yang H Z, Wang F. Mixotrophic cultivation of microalgae for biodiesel production: Status and prospects. Appl Biochem Biotechnol, 2014; 172: 3307–3329.

Scragg A H. The effect of phenol on the growth of Chlorella vulgaris and Chlorella VT-1. Enzyme Microb Technol, 2006; 39: 796–799.

Franklin N M, Stauber J L, Apte S C, Lim R P. Effect of initial cell density on the bioavailability and toxicity of copper in microalgal biomassays. Environ Toxicol Chem, 2002; 21(4): 742–751.

Harker M, Tsavalos A J, Young A J. Autotrophic growth and carotenoid production of Haematococcuspluvialis in a 30 liter air-lift photobioreactor. J Ferment Bioeng, 1996; 82: 113–118.

Hu Z Y, Li Y T, Sommerfeld M, Chen F, Hu Q. Enhanced protection against oxidative stress in an astaxanthin-over production Haematococcus mutant. Eur J Phycol, 2008; 43: 365–376.

Wang J F, Han D X, Sommerfeld M R, Lu C M, Hu Q. Effect of initial biomass density on growth and astaxanthin production of Haematococcus pluvialis in an outdoor photobioreactor. J Appl Phycol, 2013; 25: 253–260.




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