Effects of background fertilization followed by co-application of two kinds of bacteria on soil nutrient content and rice yield in Northeast China

Tao Sun, Yuyingnan Liu, Shuang Wu, Jizhou Zhang, Bin Qu, Jinggang Xu

Abstract


With the improvement of living standards, people pay more and more attention to the quality and safety of rice. Microbial agents are favored by the public because they can activate the nutrient supply in the soil, and reduce the residue and application amount of chemical fertilizers and pesticides. Based on the conventional fertilization in the field, Bacillus mucilaginosus and Aspergillus niger were applied, Bacillus mucilaginosus was inoculated at four levels in the paddy soil in the cold region of Heilongjiang Province of China. The effects of different proportions of Bacillus mucilaginosus and Aspergillus niger on the number of soil microorganisms, enzyme activity, microbial biomass, soil biochemical intensity, soil nutrient content, plant nutrient content and yield were studied, and the effects on the plant nutrient content of rice and the nutrient dynamics were discussed. The results showed that a 2.62%-21.20% higher yield of rice obtained from co-application treatments compared with that of the control-blank treatment. Furthermore, the highest yield obtained (10736±65 kg/hm2) suggested that the optimized values for the two bacteria applied were 120×1011 CFU/hm2 for Bacillus mucilaginosus and 15×1011 CFU/hm2 for Aspergillus niger. Bacillus mucilaginosus can decompose minerals in soil, dissolve potassium and silicon, decompose apatite and release phosphorus into soil. Aspergillus niger can transform the phosphate which cannot be absorbed by plants into soluble phosphate which can be directly absorbed by plants by producing non-volatile acids. In particular, Bacillus mucilaginosus and Aspergillus niger have synergistic effect, and their combined application effect is greater than that of two bacteria alone. Co-application promoted the release of soil soluble silicon, and then increased the silicon content of plants. At the same time, soil microorganism, microbial biomass, enzyme activity and biochemical activity all increased significantly. This study provides an effective way to reduce the amount of chemical fertilizer applied in rice production in cold regions of China.
Keywords: microbial inoculants, microbial fertilizer, rice, co-application, Bacillus mucilaginosus, Aspergillus niger, nutrients
DOI: 10.25165/j.ijabe.20201302.4863

Citation: Sun T, Liu Y Y N, Wu S, Zhang J Z, Qu B, Xu J G. Effects of background fertilization followed by co-application of two kinds of bacteria on soil nutrient content and rice yield in Northeast China. Int J Agric & Biol Eng, 2020; 13(2): 154–162.

Keywords


microbial inoculants, microbial fertilizer, rice, co-application, Bacillus mucilaginosus, Aspergillus niger, nutrients

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References


Zhu H J, Sun L F, Zhang Y F, Zhang X L, Qiao J J. Conversion of spent mushroom substrate to biofertilizer using a stress-tolerant phosphate-solubilizing Pichia farinose FL7. Bioresource Technology, 2012; 111: 410–416.

Zhao Z P, Yan S, Liu F, Ji P H, Wang X Y, Tong Y A. Effects of chemical fertilizer combined with organic manure on Fuji apple quality, yield and soil fertility in apple orchard on the Loess Plateau of China. Int J Agric & Biol Eng, 2014; 7(2): 45–55.

Zhao Z P, Duan M, Yan S, Liu Z F, Wang Q, Fu J, et al. Effects of different fertilizations on fruit quality, yield and soil fertility in field-grown kiwifruit orchard. Int J Agric & Biol Eng, 2017; 10(2): 162–171.

Ahmed F, Sultana R, Ahmed O, Akhtaruzzaman M, Iqbal M T. Roles of different fertilizer management practices on mulberry leaf yield and quality. Int J Agric & Biol Eng, 2017; 10(5): 104–114.

Khush G S. What it will take to feed 5.0 billion rice consumers in 2030. Plant Molecular Biology, 2005; 59(1): 1–6.

IRRI (International Rice Research Institute) (2003) World rice statistics. http://www.irri.org/science/ricestat/index.asp.

Yang Z, Chen F S, Wu X Q, Luan F G, Zhang L P, Fang X M, et al. Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments. Plos One, 2018; 13(7): e0199625.

Kumar S, Gaind S. Fermentative production of soluble phosphorus fertilizer using paddy straw: an alternate to biomass burning. International Journal of Environmental Science and Technology, 2019; 16(10): 6077–6088.

Khan K S, Joergensen RG. Changes in microbial biomass and P fractions in biogenic household waste compost amended with inorganic P fertilizers. Bioresource Technology, 2009; 100(1): 303–309.

Hinsinger P. Bioavailability of soil inorganic P in the rhizosphere as affected by root-induced chemical changes: A review. Plant and Soil, 2001; 237(2): 173–195.

Gichangi E M, Mnkeni P N S, Brookes P C. Effects of goat manure and inorganic phosphate addition on soil inorganic and microbial biomass phosphorus fractions under laboratory incubation conditions. Soil Science and Plant Nutrition, 2009; 55(6): 764–771.

Savci S. Investigation of effect of chemical fertilizers on environment. In: Dan Y, editor. International Conference on Environmental Science and Development. APCBEE Procedia, 2012; pp.287–292.

Mercl F, Tejnecký V, Ságová-Marečková M, Dietel K, Kopecký J, Břendová K, et al. Co-application of wood ash and Paenibacillus mucilaginosus to soil: the effect on maize nutritional status, root exudation and composition of soil solution. Plant and Soil, 2018; 428(1): 105–122.

Zheng G X, Zhou C Y, Yin T, Lu Z X, Ai S. Optimization of fermentation factors to enhance rice straw degradation ability using a microbial consortium LZF-12. Int J Agric & Biol Eng, 2019; 12(3): 168–173.

Gou X M, Zhang R P, Zhang Y Y, Yu W, Cai Y, Yang J W, et al. Effect of microbial fertilizers added into seedbed on microbial biomass carbon and nitrogen in rhizosphere of tobacco soil. Journal of Sichuan Agricultural University, 2019: 37(3): 338–342,351. (in Chinese)

Gamal-Eldin H, Elbanna K. Field evidence for the potential of rhodobacter capsulatus as biofertilizer for flooded rice. Current Microbiology, 2011; 62(2): 391–395.

Zhang X W. Advances in biological nitrogen fixation mechanisms. Chinese Science Bulletin, 1965:8: 705–713. (in Chinese)

Chen W X, Wang ET. Chinese rhizobia. Science Press, ISBN: 9787030297891, 2011. (in Chinese)

Fan Q S. Research and prospect of nitrogen fixing bacteria in soil in China. Acta Pedologica Sinica, 1963; 11(2): 220–228. (in Chinese)

Meng Y Xu F H, Meng Q Y, Gu W R. Current application status and prospect of microbiological fertilizer in China. Chinese Agricultural Science Bulletin, 2008: 24(6): 276–283. (in Chinese)

Yang C D. Advances in and prospects of forest soil reaserch. Acta Pedologica Sinica, 2008; 45(5): 881–891. (in Chinese)

Liu R C, Li F T Hao Z R Yang Z Y. The Development, research and popularization of biological potassium fertilizer (Silicate bacteria Inoculum). Scientia Agricultura Sinica, 1998; 31(1): 95–96. (in Chinese)

Wang C F, Xu H L, Chang T T, Wang W N. Effects of compound microbial inoculant treated wastewater irrigation on soil nutrients and enzyme activities. Int J Agric & Biol Eng, 2016; 9(6):100–108.

Sattar A, Naveed M, Ali M, Zahir Z A, Nadeem S M, Yaseen M, et al. Perspectives of potassium solubilizing microbes in sustainable food production system: A review. Applied Soil Ecology, 2019;133:146–159.

Xue D, Huang X D, Song G D, Yang R X, Wang H M, Luo Q L. Screening and growth-promoting effect of phosphate-solubilizing fungi in the rhizosphere of Paeonia suffruticosa. Ecology and Environmental Sciences, 2018; 27(9): 1639–1645. (in Chinese)

Majumder M S, Hossain M. Isolution and molecular characterization of phosphate solubilizing filamentous fungi from subtropical soils in Okinawa. Applied Ecology and Environmental Research, 2019; 17: 9145–9157.

Klaic R, Plotegher F, Ribeiro C, Zangirolami T C, Farinas C S. A fed-batch strategy integrated with mechanical activation improves the solubilization of phosphate rock by aspergillus niger. ACS Sustainable Chemistry & Engineering, 2018; 6(9): 11326–11334.

Ceci A, Pinzari F, Russo F, Maggi O, Persiani A M. Saprotrophic soil fungi to improve phosphorus solubilisation and release: In vitro abilities of several species. Ambio, 2018; 47(1): 30–40.

Zhu M L, Gong L, Zhang L L. Soil enzyme activities and their relationships to environmental factors in a typical oasis in the upper reaches of the Tarim River. Environmental Science, 2015; 36(7): 2678–1685. (in Chinese)

Lin X G. Principles and methods of soil microbiology research. Beijing: Higher Education Press, ISBN: 9787040284072, 2010. (in Chinese)

Wu J S, Lin Q M, Huang Q Y, Xiao H A. Method and application of soil microbial biomass measurement. Beijing: China Meteorological Press, ISBN: 9787502941581, 2006. (in Chinese)

Guan S Y. Soil enzymes and their research methods. Beijing: China Agricultrure Press, ISBN: 16144•3123, 1986. (in Chinese)

Bao S D. Soil agro-chemistrical analysis. Beijing: China Agricultrure Press; ISBN: 9787109066441, 2008. (in Chinese)

Tan Z J, Feng Y H, Liu F, Zhang Y Z, Zou Y B. Effects of rice-based cropping system and organic manure on microbes and enzyme activities in paddy soils derived from red earth. Chinese Journal of Eco-Agriculture, 2004; 12(2): 121–123. (in Chinese)

Wu N, Pan H-X, Qiu D, Zhang Y M. Feasibility of EPS-producing bacterial inoculation to speed up the sand aggregation in the Gurbantunggut Desert, Northwestern China. Journal of Basic Microbiology, 2014; 54(12): 1378–1386.

Piekarska K, Trusz A, Szczesniak S. Bacteria and fungi in two air handling units with air recirculating module. Energy and Buildings, 2018; 178: 154–164.

Wu Y, Hou L, Zhang J. Antagonistic effect of bacterial strains in complex microbial inoculants and the screening of their culture condition. Acta Agriculturae Zhejiangensis, 2016; 28(5): 820–827. (in Chinese)

Schweigert M, Herrmann S, Miltner A, Fester T, Kaestner M. Fate of ectomycorrhizal fungal biomass in a soil bioreactor system and its contribution to soil organic matter formation. Soil Biology & Biochemistry, 2015; 88: 120–127.

Mahanta K, Jha D K, Rajkhowa D J, Manoj K. Microbial enrichment of vermicompost prepared from different plant biomasses and their effect on rice (Oryza sativa L.) growth and soil fertility. Biological Agriculture & Horticulture, 2012; 28(4): 241–250.

Ge T, Liu C, Yuan H, Zhao Z, Wu X, Zhu Z, et al. Tracking the photosynthesized carbon input into soil organic carbon pools in a rice soil fertilized with nitrogen. Plant and Soil, 2015; 392(1-2): 17–25.

Cao Z, Li D, Han X. The fungal to bacterial ratio in soil food webs, and its measurement. Acta Ecologica Sinica, 2011; 31(16): 4741–4748. (in Chinese).

Tian X, Li J, Wang C, Chu G, Wei C, Zheng Q, et al. Effects of continuous application of bio-organic fertilizer for three years on soil nutrients, microbial biomass and enzyme activity. Soils, 2014; 46(3): 481–488. (in Chinese)

Guo R, Xia Z Z. Effect of continuous fertilization on biochemistry intensity of Lou soil. Journal of Anhui Agricultural Sciences, 2008; 36(27): 11859–11860, 11889. (in Chinese)

Zhang W, Wang B. Study on phosphate-solubilizing activity and suitable conditions of a strain of bacillus mucilaginosus. Chinese Agricultural Science Bulletin, 2014; 30(21): 136–140. (in Chinese)

Hou W, Yang L, Chen R, Zhang S. Effects of aspergillus niger phyA2 transgenic maize on utilization of organic phosphorus in soil. Acta Agronomica Sinica, 2013; 39(8): 1360–1365. (in Chinese)

Hu J, Yu J P, Lian B. Capability and mechanism of potassium releasing from potassium-bearing minerals by aspergillus niger. Bulletin of Mineralogy Petrology and Geochemistry, 2011; 30(3): 277–285. (in Chinese)

Biswas D R, Basak B B. Mobilization of potassium from waste mica by potassium-solubilizing bacteria (bacillus mucilaginosus) as influenced by temperature and incubation period under in vitro laboratory conditions. Agrochimica, 2014; 58(4): 309–320.

Zhuang Z J, Du Y H, Zhu R Y, Zhang J H. Effects of silicate bacterium on biological character and nutrients accumulation of rice. China Rice, 2015; 21(6): 88–90. (in Chinese)

Zhang X Q, Ren H J, Liu N, Zhang L Y, Zhou R. Changes in texture and retorting yield in oil shale during its bioleaching by bacillus mucilaginosus. Chemical Research in Chinese Universities, 2013; 29(2): 294–298.

Meena V S, Maurya B R, Verma J P. Does a rhizospheric microorganism enhance K+ availability in agricultural soils? Microbiological Research, 2014; 169(5-6): 337–347.

Karavaiko G I, Krutsko V S, Mel'nikova E O, Avakian Z A, Ostroushko I I. Role of microorganisms in the destruction of spodumene. Mikrobiologiia, 1980; 49(3): 547–551.

Gaind S. Effect of fungal consortium and animal manure amendments on phosphorus fractions of paddy-straw compost. International Biodeterioration & Biodegradation, 2014; 94: 90–97.

Abou-el-Seoud I I, Abdel-Megeed A. Impact of rock materials and biofertilizations on P and K availability for maize (Zea Maize) under calcareous soil conditions. Saudi Journal of Biological Sciences, 2012; 19(1): 55–63.

Zhang L, Fan J, Niu W, Li T, Wu R, Jing Y, et al. Isolation of phosphate solubilizing fungus (aspergillus niger) from caragana rhizosphere and its potential for phosphate solubilization. Acta Ecologica Sinica, 2011; 31(24):7571–7578. (in Chinese)

Kang S-M, Waqas M, Shahzad R, You Y-H, Asaf S, Khan MA, et al. Isolation and characterization of a novel silicate-solubilizing bacterial strain Burkholderia eburnea CS4-2 that promotes growth of japonica rice (Oryza sativa L. cv. Dongjin). Soil Science and Plant Nutrition, 2017; 63(3): 233–241.

Ma J F, Yamaji N. Silicon uptake and accumulation in higher plants. Trends in Plant Science, 2006; 11(8): 392–397.

Mitani N, Ma J F. Uptake system of silicon in different plant species. Journal of Experimental Botany, 2005; 56(414): 1255–1261.

Liang Y, Si J, Römheld V. Silicon uptake and transport is an active process in Cucumis sativus. New Phytologist, 2005; 167(3): 797–804.

Li X, Wu Z, Li W, Yan R, Li L, Li J, et al. Growth promoting effect of a transgenic bacillus mucilaginosus on tobacco planting. Applied Microbiology and Biotechnology, 2007; 74(5): 1120–1125.

Han H S, Supanjani, Lee K D. Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant Soil and Environment, 2006; 52(3): 130–136.

Hu Z, Wu Y, Mao Z, He Y. Isolation, identification and biological characterization of silicate bacteria. Acta Agriculturae Universitatis Jiangxiensis, 2013; 35(3): 609–614. (in Chinese)

Wu J, Xie F, Fu T, Pan X, Shi Q. Effect of application of nitrogen combined with silicon on grain yield of double cropping rice and its nitrogen and silicon absorption. Acta Agriculturae Universitatis Jiangxiensis, 2017; 39(5): 843–850. (in Chinese)

Pati S, Pal B, Badole S, Hazra G C, Mandal B. Effect of silicon fertilization on growth, yield, and nutrient uptake of rice. Communications in Soil Science and Plant Analysis, 2016; 47(3): 284–290.

Ogut M, Er F, Neumann G. Increased proton extrusion of wheat roots by inoculation with phosphorus solubilising microorganims. Plant and Soil, 2011; 339(1-2): 285–297.

Sun Y, Wang X, Wang Y, Pan W, Lu R, Ruan Z, et al. Soil available nutrients and rice yield with silicon and phosphorus fertilization. Journal of Zhejiang University, 2015; 32(4): 551–556. (in Chinese)

Agostinho F B, Tubana B S, Martins M S, Datnoff L E. Effect of different silicon sources on yield and silicon uptake of rice grown under varying phosphorus rates. Plants-Basel, 2017; 6(3): 35–52.




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