LabVIEW-based impedance biosensing system for detection of avian influenza virus

Zhang Benhua, Ronghui Wang, Yixiang Wang, Yanbin Li

Abstract


In order to detect the multiple avian influenza viruses (AIVs) rapidly, specifically and sensitively, a LabVIEW and microelectrode array-based impedance biosensor was developed and demonstrated. A laptop with LabVIEW software was used to generate excitation signals at different frequencies with an audio card and measure the impedance of target viruses through a data acquisition card. The audio card of the laptop was used as a function generator, while a data acquisition card was used for data communication. A virtual instrument was programmed with LabVIEW to provide a platform for impedance measurement, data processing, and control. Six interdigitated microelectrodes were placed at the bottom of six wells on a microplate to form six sensors for different AIVs and controls. Then, AIV specific ligands were immobilized on the microelectrode surface to capture target viruses. To enhance the sensitivity, AIV specific aptamers conjugated gold nanoparticles and thiocyanuric acid were employed to form a network structure and used as an amplifier. Results of the measured impedance were compared with a commercial IM6 impedance analyzer, and the error was less than 5%. The developed biosensor was portable with the sensitivity and specificity for applications to on-site or in-field rapid screening of avian influenza viruses.
Keywords: biosensor, impedance detection, avian influenza virus, LabVIEW
DOI: 10.3965/j.ijabe.20160904.1704

Citation: Zhang B H, Wang R H, Wang Y X, Li Y B. LabVIEW-based impedance biosensing system for detection of avian influenza virus. Int J Agric & Biol Eng, 2016; 9(4): 116－122.

Keywords


biosensor, impedance detection, avian influenza virus, LabVIEW

Full Text:

PDF

References


WHO, http://www.who.int/influenza/human_animal_interface/ EN_GIP_20141204CumulativeNumberH5N1cases.pdf., Accessed on [2014-12-04].

WHO, Overview of the emergence and characteristics of the avian influenza A (H7N9) virus. May 31, 2013.

WHO, Influenza research at the human and animal interface. Report of a WHO working group. September 21-22, 2006, Geneva, Switzerland.

Bai H, Wang R, Hargis B, Lu H, Li Y. A SPR aptasensor for detection of avian influenza virus H5N1. Sensors, 2012; 12: 12506-12518.

Wang R, Li Y. Hydrogel based QCM aptasensor for detection of avian influenza virus. Biosens. Bioelectron., 2013; 42: 148–155.

Zhang Y, Deng Z, Yue J, Tang F, Wei Q. Using cadmium telluride quantum dots as a proton flux sensor and applying to detect H9 avian influenza virus. Anal. Biochem., 2007; 364: 122–127.

Xu J, Suarez D, Gottfried D. Detection of avian influenza virus using and interferometric biosensor. Anal. Bioanal. Chem., 2007; 389: 1193–1199.

Qi C, Tian X, Chen S, Yan J, Cao Z, Tian K, et al. Detection of avian influenza virus subtype H5 using biosensor based on imaging ellipsometry. Biosens. Bioelectron., 2012; 25: 456–460.

Lai W A, Lin C H, Y. S. Yang, M. S. Lu. Ultrasensitive and label-free detection of pathogenic avian influenza DNA by using CMOS impedimetric sensors. Biosens. Bioelectron., 2010; 35: 1530–1534.

Bolis S D, Charalambous P C, Efstathiou C E, Mantzila A G, Malamou C A, Prodromidis M I. Monitoring of the avidin–biotylinated dextran interaction on Au- and Ti/TiO2-electrode surfaces using a charge integrating device. Sens. Actuat. B, 2006; 114: 47–57.

Wang R, Zhao J, Jiang T, Kwon Y M, Lu H, Jiao P, et al. Selection and characterization of DNA aptamers for use in detection of avian influenza virus H5N1. J. Virol. Meths., 2013; 189: 362–369.

Lum J, Wang R, Lassiter K, Srinivasan B, Abi-Ghanem D, Berghman, et al. Rapid detection of avian influenza H5N1 virus using impedance measurement of immuno-reaction coupled with RBC amplification. Biosens. Bioelectron, 2011; 38(1): 67–73.

Ram Y, Yoetz-Kopelman T, Dror Y, Freeman A, Shacham-Diamand Y. Impact of molecular surface charge on biosensing by electrochemical impedance spectroscopy. Electrochimica Acta, 2016; 200: 161–167.

Lu Y, Guo Z, Song J J, Huang Q A, Zhu S W, Huang X J, et al. Tunable nanogap devices for ultra-sensitive electrochemical impedance biosensing. Analytica Chimica Acta, 2016; 905: 58–65.

Wu C C, Huang W C, Hu C C. An ultrasensitive label-free electrochemical impedimetric DNA biosensing chip integrated with a DC-biased AC electro osmotic vortex. Sensors and Actuators B: Chemical, 2015; 209: 61–68.

Chen Y, Wang J, Liu Z M. Graphene and Its Derivative-based Biosensing Systems. Journal of Analytical Chemistry, 2012; 40(11): 1772–1779.

Cao X D, Ye Y K, Liu S Q. Gold nanoparticle-based signal amplification for biosensing. Analytical Biochemistry, 2011; 417: 1–16.

Heileman K, Daoud J, Tabrizian M. Dielectric spectroscopy as a viable biosensing tool for cell and tissue characterization and analysis. Biosensors and Bioelectronics, 2013; 49: 348–359.

Kim S G, Lee H J, Lee J H, Jung H I, Yook J G. A highly sensitive and label free biosensing platform for wireless sensor node system. Biosensors and Bioelectronics, 2013; 50: 362–367.

Bonanni A, Loo A H, Pumera M. Graphene for impedimetric biosensing. TrAC Trends in Analytical Chemistry, 2012; 37: 12–21.

Voiculescu I, Nordin A N. Acoustic wave based MEMS devices for biosensing applications. Biosensors and Bioelectronics, 2012; 33: 1–9.

Kashefi-Kheyrabadi L, Mehrgardi M A. Design and construction of a label free aptasensor for electrochemical detection of sodium diclofenac. Biosensors and Bioelectronics, 2012; 33: 184–189.




Copyright (c)



2023-2026 Copyright IJABE Editing and Publishing Office