Extraction, quantification and characterization of oil from pumpkin seeds

Tonny Kukeera, Noble Banadda, Peter Tumutegyereize, Nicholas Kiggundu, Ratibu Asuman

Abstract


A quantification and characterization study of oil from pumpkin seeds was carried out on three pumpkin varieties, namely, Japanese type of the Curcubita maxima species, Green Kabocha and butternut squash of the Cucurbita moschanta species. Oil extraction was done using the Soxhlet method with petroleum ether as the solvent. The physicochemical properties of acid value, iodine value, specific gravity and refractive index were determined. The results were analyzed statistically using one way ANOVA at a 5% level of significance. The mean values of oil content, acid value, iodine value, specific gravity and refractive index for Japanese type pumpkin were found to be 35.67%, 2.24 mg KOH/g, 26.45 mg I2/100 g, 0.9126 and 1.47, respectively. For Green Kabocha, the mean values of the above five properties were 30.12%, 3.35 mg KOH/g, 25.3 mg I2/100 g, 0.9126 and 1.469, respectively. For the Butternut squash type the mean values of the above five properties were 34.76%, 3.74 mg KOH/g, 26.61 mg I2, 0.9131 and 1.471, respectively. It can be noted that from the low acid values of the pumpkin oil, pumpkin oil can be identified as edible. Also the low iodine value indicated that the pumpkin oil was non-drying. In addition, the low iodine value also suggested that the oil contains few unsaturated bonds and therefore has low susceptibility to oxidative rancidity. With a comparison of extraction rates from other oil sources in Uganda such as cotton (15%-20%), soybeans (19%-21%), the obtained results in this study suggested that pumpkin seeds are a viable source of vegetable oils that can be utilized for commercial vegetable oil extraction in Uganda.

DOI: 10.3965/j.ijabe.20150801.013

Citation: Kukeera T, Banadda N, Tumutegyereize P, Kiggundu N, Asuman R. Extraction, quantification and characterization of oil from pumpkin seeds. Int J Agric & Biol Eng, 2015; 8(1): 98-102.

Keywords


pumpkin seeds, pumpkin varieties, oil extraction, physicochemical properties, vegetable oil, characterization

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References


Lee Y K, Chung W I, Ezura H. Efficient plant regeneration via organogenesis in winter squash (Cucurbita maxima Duch.). Plant Science, 2003; 164: 413–418.

Lineback N, Gritzner M L. Geography in the News: Pumpkins. 2013. Available at: www.newswatch. nationalgeographic.com. Accessed on [2014-10-20].

Echessa A C P, Nyambaka H, AOndigi N, Omuterema S, Toili W, Sande A. Variation of micronutrients in pumpkin fruit varieties grown within the Lake Victoria Basin. Food Science and Quality Management, 2013; 17, 33–40.

Esuoso K, Lutz H, Kutubuddin M, Bayer E. Chemical composition and potential of some underutilized tropical biomass. I: fluted pumpkin (Telfairia occidentalis). Food Chemistry, 1998; 61: 487–492.

Ibrahim A, Onwualu A P. Technologies for extraction of oil bearing agricultural products: A review. Journal of Agricultural Engineering and Technology (JAET), 2005; 13, 23–32.

Alfawaz M A. Chemical composition and oil characteristics of pumpkin (Cucurbita maxima) seed kernels. Food Science and Agriculture, 2004; 2(1): 5–18.

Fu C, Shi H, Li Q. A review on pharmacological activities and utilization technologies of pumpkin. Plant Foods for Human Nutrition, 2006; 61(2): 73–80.

Jakab A, Jablonkai I, E Forga’cs. Quantification of the ratio of positional isomer dilinoleoyl-oleoyl glycerols in vegetable oils. Rapid Commun. Mass Spetrom, 2003; 17(20): 2295–2302.

Stevenson D G, Eller F J, Wang L, Jane J, Wang T, Inglett G E. Oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. Journal of Agricultural and Food Chemistry, 2007; 55: 4005–4013.

Zhang X, Ouyang J Z, Zhang Y S, Tayalla B, Zhou X C, and Zhou S W. Effect of the extracts of pumpkin seeds on the urodynamics of rabbits: an experimental study. Journal of Tongji Medical University, 1994; 14(4): 235–238.

Haque M, Islam M, Hussain M, Khan F. Physical, mechanical properties and oil content of selected indigenous seeds available for biodiesel production in Bangladesh. Agricultural Engineering International: CIGR Journal, 2010.

Nwokolo E, Sim J S. Nutritional assessment of defatted oil meals of melon (Colocynthis citrullus L.) and fluted pumpkin (Telfaria occidentalis Hook) by chick assay. Journal of the Science of Food and Agriculture, 1987; 38: 237–246.

AOAC. Official methods of analysis (16th Ed.). Washington, DC: Association of Official Analytical Chemists, USA, 1995.

Shukla B D, Srivastava P K, Gupta R K. Oil Seeds Processing Technology. Indian Council of Agricultural Research, Delhi Delhi. 1992.

Mabaleha M, Mitei Y, Yeboah S. A comparative study of the properties of selected melon seed oils as potential candidates for development into commercial edible vegetable oils. Journal of the American Oil Chemists’ Society, 2007; 84(1): 31–36.

Bwade K E, B Aliyu, Kwaji A M. Physicochemical properties of pumpkin seed oil relevant to bio-diesel production and other industrial applications. International Journal of Engineering, Business and Enterprise Applications (IJEBEA), 2013; 4(1) 72–78.

Akinoso R, Raji A O. Optimization of oil extraction from locust bean using response surface methodology. European Journal of Lipid Science and Technology, 2011; 113(2): 245–252.

Onimawo I A. Proximate composition and selected physico chemical properties of the seed, pulp and oil of Soar Soap (Annona muricata). Plant Foods for Human Nutrition, 2002; 7(4): 570–573.

Obasi N A, Ukadilonu J, Eze E, Akubugwo E I, Okorie U C. Proximate composition, extraction, characterization and comparative assessment of coconut (Cocos nucifera) and melon (Colocynthis citrullus) seeds and seed oils. Pakistan Journal of Biological Sciences, 2012; 15(1): 1–9.

Earl G H, Lawrence A J, Su C, Tong W, Pamela J W. Soybean oil. Bailey’s Industrial Oil and Fat Products. John Wiley & Sons, Inc. 2005.

Orhevba B A, Efomah A N. Extraction and characterization of cottonseed (gossypium) oil. International Journal of Basic and Applied Science, 2012; 1: 398–402.

Huang X E, Hirose K, Wakai K, Matsuo K, Ito H, Xiang J, et al. Comparison of lifestyle risk factors by family history for gastric, breast, lung and colorectal cancer. Asian Pacific Journal of Cancer Prevention, 2004; 5(4): 419–427.




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